mouse monoclonal anti dmpk  (Developmental Studies Hybridoma Bank)

Journal: International Journal of Molecular Sciences

Article Title: Nuclear Envelope Alterations in Myotonic Dystrophy Type 1 Patient-Derived Fibroblasts

doi: 10.3390/ijms23010522

Figure Lengend Snippet: Primary antibodies used to detect the multiple proteins analyzed by Western blotting and immunocytochemistry.

Article Snippet: Mouse monoclonal anti-DMPK (MANDM1 6G8) , Developmental Studies Hybridoma Bank , WB—0.2 ug/mL.

Techniques: Western Blot, Immunocytochemistry

Journal: PLoS ONE

Article Title: Aldosterone signaling regulates the over-expression of claudin-4 and -8 at the distal nephron from type 1 diabetic rats

doi: 10.1371/journal.pone.0177362

Figure Lengend Snippet: SPL decreased diabetes-induced cldn-4 and -8 expressions evaluated by IF, IB and qRT- PCR. IF analysis shows that 3 weeks after diabetes induction, increased expressions of (A) cldn-4 (green label) and (E) cldn-8 (green label) were found in the TJs of DT labeled with desmoplakin (DMPK, red label). Nuclei were marked with DAPI (blue label). SPL treatment significantly decreased these changes. To confirm IF findings, IB and qRT-PCR analyzes were performed . Diabetic condition significantly increased protein (B) and mRNA (D) levels of cldn-4; these changes were decreased by SPL treatment. Also, SPL treatment decreased diabetes-induced increased protein (F) and mRNA (H) levels of cldn-8. Similar results were found between CTL and SPL groups. GAPDH was used as loading control. Densitometric analyzes of IB from the four experimental groups are shown in panels C for cldn-4 and, G for cldn-8 . Data are mean±SEM from 3 rats per group. *p<0.05; **p<0.01 and ***p<0.001. Images are representative of three different experiments performed in the four experimental groups. Bar = 20 μm.

Article Snippet: Mouse anti-desmoplakin (DMPK) antibody was purchased from MP Biomedicals (Solon, OH, USA).

Techniques: Quantitative RT-PCR, Labeling